Monoclonal polyhisitidine antibodies (6xHis-tag)

Polyhistidine tags are used, for example, to purify recombinant proteins by affinity chromatography. The anti-polyhistidine tag antibodies are suitable, for example, for the detection of the His tag on these recombinant proteins.

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against polyhistidine tags (6xHis-tag).

Monoclonal Legionella antibodies

Legionella are gram-negative, aerobic bacteria. According to the RKI, over 60 species with almost 80 different serogroups are known. Legionella can multiply inside cells, especially in water in free-living amoebae, but also in human macrophages and are therefore facultative intracellular parasites. Legionella find ideal growth conditions at temperatures between 25 and 45° C.

In principle, all Legionella are to be classified as potentially pathogenic to humans. In Europe, approx. 90 % of illnesses are caused by Legionella pneumophila serogroup 1 (approx. 80 %) and serogroups 4 and 6 (together approx. 20 %). (Source: RKI).

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against different serogroups of Legionella pneumophila.

Detection of methicillin-resistant S. aureus

Methicillin-resistant S. aureus strains (MRSA) are characterized by the fact that they are resistant not only to all β-lactam antibiotics currently available on the market, but also to the penicillase-stable antibiotics methicillin and oxacillin. The cause of this resistance lies in the mecA gene, which encodes a modified penicillin-binding protein (PBP2a). An infection can cause serious illnesses such as pneumonia, bone or heart valve infections, especially in weakened people.

Resistance marker penicillin binding protein 2a (PBP2a)

PBP2a stands for penicillin-binding protein 2a and is responsible for resistance to beta-lactam antibiotics. PBP2a confers resistance to methicillin, penicillin and related ß-lactam antibiotics.

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against penicillin-binding protein 2a (PBP2a).

The antibodies produced by the hybridoma cell lines PBP-003 and PBP-012 together form a matched pair. This pair of capture and detector antibodies binds to different epitopes of the protein and thus enables specific detection of the resistance marker PBP2a.

Virulence marker Panton-Valentine leukocidin (PVL)

Panton-Valentine leukocidin (PVL) is a strong virulence factor of Staphylococcus aureus. This exotoxin leads to the lysis of leukocytes by forming pores in the cytoplasmic membrane. PVL-producing S. aureus pathogens are highly pathogenic and cause severe infections of the skin and soft tissue, including lethal pneumonia.

The antibodies produced by the hybridoma cell lines LUK-002 and LUK-003 together form a matched pair. The two antibodies bind specifically to different regions of the PVL toxin and thus enable high sensitivity and specificity in the identification of PVL-expressing S. aureus strains.

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against Panton-Valentine leukocidin (PVL).

Detection of paratuberculosis

Paratuberculosis (Johne’s disease) is caused by the Mycobacterium avium ssp. paratuberculosis (MAP). It occurs predominantly in ruminants and can lead to chronic inflammatory bowel disease. We have developed a set of antibodies for the immunological detection of paratuberculosis.

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against Mycobacterium avium ssp. paratuberculosis (MAP).

Detection of mycotoxins

Mycotoxins (mold toxins) are metabolic products formed by fungi that can be found in agricultural products and foodstuffs. Mycotoxins can have various toxic effects on vertebrates even in very small quantities. A disease caused by this is known as mycotoxicosis. The most frequently occurring mycotoxins are the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) produced by the genus Fusarium.

Overview antibodies & hybridoma cell lines

We offer the following monoclonal antibodies and AK-producing hybridoma cell lines against mycotoxins are the mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) produced by the genus Fusarium.